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27 February 2007 Single quantum dot fluorescence resonant energy transfer: probing the heterogeneity in macroscopic samples
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Abstract
We present a single QD FRET study that allows us to probe the heterogeneity in QD-dye labeled protein conjugates. We first show that QDs are compatible with spFRET detection by demonstrating the equivalence of single particle and ensemble measurement modalities in terms of derived average FRET efficiencies and separation distances between a QD donor and dyes attached to specific sites on conjugated proteins. We then use spFRET data to demonstrate that the valence distribution of QD-protein conjugates follows Poisson statistics.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Thomas Pons, Igor L. Medintz, Xiang Wang, Douglas S. English, and Hedi Mattoussi "Single quantum dot fluorescence resonant energy transfer: probing the heterogeneity in macroscopic samples", Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 64480I (27 February 2007); https://doi.org/10.1117/12.717539
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