Whole-body subcellular multicolor imaging

Meng Yang; Ping Jiang; Robert M. Hoffman

doi:10.1117/12.714035

3 February 2007 Whole-body subcellular multicolor imaging

Meng Yang, Ping Jiang, Robert M. Hoffman

Proceedings Volume 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV; 64490V (2007) https://doi.org/10.1117/12.714035
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

An important goal for in vivo imaging is to be able to non-invasively image single cells. The Olympus IV100 Laser Scanning Microscope, with ultra-thin microscope objectives ("stick objectives"), was used for three-color whole-body imaging of individual two-color cancer cells interacting with the GFP-expressing stromal cells. Cellular dynamics were non-invasively imaged including mitotic and apoptotic tumor cells, stromal cells interacting with the tumor cells, tumor vasculature, and tumor blood flow. This imageable model should lead to a new paradigm of in vivo cancer cell biology and to new visible real-time targets for cancer drug discovery.

Citation Download Citation

Meng Yang, Ping Jiang, and Robert M. Hoffman "Whole-body subcellular multicolor imaging", Proc. SPIE 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV, 64490V (3 February 2007); https://doi.org/10.1117/12.714035

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