13 March 2007 Attenuation of molecular function by multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI) using green fluorescent protein
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Abstract
Noninvasive and straightforward methods to inactivate selected proteins in the living cell with high spatiotemporal resolution are eagerly sought for elucidation of protein function in the post-genome-mapping era. Chromophore-assisted laser inactivation (CALI) facilitates inactivation of proteins by photochemically generated reactive oxygen species (ROS), but CALI using single-photon excitation thus far has presented several drawbacks, including its complex procedure, low efficiencies of inactivation with a certain chromophore, and photodamage effects. We here show that by application of multiphoton excitation to CALI using near-infrared femtosecond laser, enhanced green fluorescent protein (EGFP) can work as an effective chromophore for inactivation of a protein's function without nonspecific photodamage in the living cell.
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Tetsuro Takamatsu, Tetsuro Takamatsu, } "Attenuation of molecular function by multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI) using green fluorescent protein", Proc. SPIE 6460, Commercial and Biomedical Applications of Ultrafast Lasers VII, 646004 (13 March 2007); doi: 10.1117/12.709235; https://doi.org/10.1117/12.709235
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