13 March 2007 Development of protein crystallization and processing: femtosecond laser, all solid-state 193 nm laser, and solution stirring techniques
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Abstract
We have developed novel techniques of protein crystallization and processing using femtosecond laser, all solid-state 193 nm laser, and solution stirring. Femtosecond laser technique enables us to extremely increase the crystallization probability, and to trigger the nucleation at low supersaturation of solution where spontaneous nucleation dose not occur. Since the laser-induced nuclei grow slowly in the low-range supersaturated solution, the crystals exhibit high crystallinity. The processing techniques using femtosecond laser and all solid-state 193 nm laser are effective for processing and manipulation of protein crystals without significant damage. Solution stirring technique also contributes the improvement of the crystal quality. We have succeeded in obtaining high-quality crystals of various proteins using the techniques, and revealing the precise structure of the protein molecules from the X-ray analysis. These techniques can accelerate structural biology and subsequent structure-based drug discoveries, resulting in important revelations in these fields.
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Yusuke Mori, Kazufumi Takano, Hiroaki Adachi, Tsuyoshi Inoue, Satoshi Murakami, Hiroyoshi Matsumura, Masafumi Kashii, Hiroshi Y. Yoshikawa, Syou Maki, Tomoya Kitatani, Shino Okada, Takatomo Sasaki, "Development of protein crystallization and processing: femtosecond laser, all solid-state 193 nm laser, and solution stirring techniques", Proc. SPIE 6460, Commercial and Biomedical Applications of Ultrafast Lasers VII, 646008 (13 March 2007); doi: 10.1117/12.714332; https://doi.org/10.1117/12.714332
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