22 January 2007 Methods and instruments for continuous-flow PCR on a chip
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Abstract
PCR is the most commonly used method to identify DNA segments. Several methods have been used to miniaturize PCR and perform it in a microfluidic chip. A unique approach is the continuous-flow PCR, where the conventional thermocycling is replaced by pumping the sample through a channel which meanders over stationary temperature zones, allowing fast temperature changes in the sample due to the low thermal mass as well as a continuous production of PCR products. In this paper we present a system comprising a polymer microfluidic chip, a thermocycler unit and the protocols necessary to perform fast continuous-flow PCR including experimental results in comparison with a conventional PCR system.
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Claudia Gärtner, Claudia Gärtner, Richard Klemm, Richard Klemm, Holger Becker, Holger Becker, "Methods and instruments for continuous-flow PCR on a chip", Proc. SPIE 6465, Microfluidics, BioMEMS, and Medical Microsystems V, 646502 (22 January 2007); doi: 10.1117/12.713797; https://doi.org/10.1117/12.713797
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