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22 January 2007 A hyper-step DNA computing system based on surface plasmon resonance
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Abstract
We reported a reusable DNA computing platform for solving satisfiability (SAT) problem based on surface plamon resonance (SPR) technology in this paper. Three different sequences of 18-mer ssDNAs with thiol terminal were first immobilized on the gold surface and then hybridized with their complementary sequences at specific sites via microfluidic channels under room temperature. We also conjugated monoclonal antibody (human IgG) to these complementary pairs chemically to amplify the hybridization signal and thus enhance the noise margin to distinguish Boolean value of true and false. In order to keep the reaction temperature and SPR measurement stable, repeated DNA annealing and denaturing is doned by varying salt concentration (by adding NaOH to denature DNA) of reaction solution rather than changing reaction temperature. The experimental results successfully demonstrated a multi-channel microfluidic DNA computation system to solve a three variables (X, Y, Z) Boolean SAT problem (formula) with reusability and specificity using protein-ssDNA conjugates to link to complementary ssDNA SAM surface under room temperature within one hour. This technique provide a feasible solution to miniaturize the DNA computation platform for possible iterated hyperstep computing processes.
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Tsung-Yao Chang, Che-Hsin Lin, Chia-Ning Yang, and Chii-Wann Lin "A hyper-step DNA computing system based on surface plasmon resonance", Proc. SPIE 6465, Microfluidics, BioMEMS, and Medical Microsystems V, 646505 (22 January 2007); https://doi.org/10.1117/12.702108
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