An integrated microsystem for multiplex processing of encoded silicon microbeads

Daniel Hoffmann; Maeve Curtin; Michael Loughran

doi:10.1117/12.702565

22 January 2007 An integrated microsystem for multiplex processing of encoded silicon microbeads

Daniel Hoffmann, Maeve Curtin, Michael Loughran

Proceedings Volume 6465, Microfluidics, BioMEMS, and Medical Microsystems V; 64650D (2007) https://doi.org/10.1117/12.702565
Event: MOEMS-MEMS 2007 Micro and Nanofabrication, 2007, San Jose, California, United States

Abstract

A novel integrated microsystem for multiplex processing of encoded microbeads on a single microchip is presented. Conventional bio-analysis of proteins and DNA requires a combination of different techniques including: accurate delivery of reagents, mixing, then reaction at controlled temperature to yield a detectable product. Standard laboratory bio-assays require intervention at several stages to manipulate samples. Furthermore, ultra sensitive quantification with a colorimetric or fluorescent label is required to obtain the necessary results. This process is time consuming and labour intensive. However the new multiplex microsystem reported here enhances logical bio-assay development due to the integration of a compact optical detection system with customized analysis software in an enclosed microfluidic environment. The bioassay was realised by careful integration of a Peltier cell and associated control electronics which enabled specific identification of a hybridized DNA sequence from a 4 x 4 cDNA library at a fixed temperature of 42^oC. The multiple fluorescence measurements of complementary hybridised DNA at the surface of encoded microbeads was confirmed. Thus, the complete integration of the different bio-assay components on a single multiplex assay platform provides distinct advantages of reduced sample volume, rapid analysis and low cost.

Citation Download Citation

Daniel Hoffmann, Maeve Curtin, and Michael Loughran "An integrated microsystem for multiplex processing of encoded silicon microbeads", Proc. SPIE 6465, Microfluidics, BioMEMS, and Medical Microsystems V, 64650D (22 January 2007); https://doi.org/10.1117/12.702565

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