22 January 2007 An integrated microsystem for multiplex processing of encoded silicon microbeads
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A novel integrated microsystem for multiplex processing of encoded microbeads on a single microchip is presented. Conventional bio-analysis of proteins and DNA requires a combination of different techniques including: accurate delivery of reagents, mixing, then reaction at controlled temperature to yield a detectable product. Standard laboratory bio-assays require intervention at several stages to manipulate samples. Furthermore, ultra sensitive quantification with a colorimetric or fluorescent label is required to obtain the necessary results. This process is time consuming and labour intensive. However the new multiplex microsystem reported here enhances logical bio-assay development due to the integration of a compact optical detection system with customized analysis software in an enclosed microfluidic environment. The bioassay was realised by careful integration of a Peltier cell and associated control electronics which enabled specific identification of a hybridized DNA sequence from a 4 x 4 cDNA library at a fixed temperature of 42oC. The multiple fluorescence measurements of complementary hybridised DNA at the surface of encoded microbeads was confirmed. Thus, the complete integration of the different bio-assay components on a single multiplex assay platform provides distinct advantages of reduced sample volume, rapid analysis and low cost.
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Daniel Hoffmann, Daniel Hoffmann, Maeve Curtin, Maeve Curtin, Michael Loughran, Michael Loughran, } "An integrated microsystem for multiplex processing of encoded silicon microbeads", Proc. SPIE 6465, Microfluidics, BioMEMS, and Medical Microsystems V, 64650D (22 January 2007); doi: 10.1117/12.702565; https://doi.org/10.1117/12.702565

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