22 January 2007 Cross-polarization scheme for fluorescence detection for biochip and biomedical applications
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The trend in medical equipment is toward compact and integrated low cost medical test devices. Fluorescence-based assays are used to identify specific pathogens through the presence of dyes, but typically require specialized microscopes and narrow-band optical filters to extract information. We present a novel method of using polarizers in cross orientation with each other to filter out excitation light and allow detection of low signal levels of fluorescence with a simple intensity-based detector in the presence of high levels of excitation light. This concept is demonstrated using an inverted microscope fitted with a halogen lamp as the excitation source and an organic photovoltaic (PV) cell as the intensity detector. The excitation light is linearly polarized and used to illuminate a microfluidic device containing a 50µl volume of Rhodamine 6G dye dissolved in water. The detector (with a second polarizer orientated perpendicularly to the first) is placed over the microfluidic device. The resulting emission signal was detected by the organic PV cell down to a concentration of 100 nM This suggests that an integrated microfluidic device, with a PV detector and an organic light emitting excitation source and integrated polarizers, could be fabricated to realize a economical "lab on a chip" device for fluorescence assays.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Andrea Pais, Andrea Pais, Haichuan Mu, Haichuan Mu, Erik Peterson, Erik Peterson, Ansuman Banerjee, Ansuman Banerjee, Ian Papautsky, Ian Papautsky, David Klotzkin, David Klotzkin, "Cross-polarization scheme for fluorescence detection for biochip and biomedical applications", Proc. SPIE 6465, Microfluidics, BioMEMS, and Medical Microsystems V, 64650M (22 January 2007); doi: 10.1117/12.720741; https://doi.org/10.1117/12.720741


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