22 January 2007 Cross-polarization scheme for fluorescence detection for biochip and biomedical applications
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Abstract
The trend in medical equipment is toward compact and integrated low cost medical test devices. Fluorescence-based assays are used to identify specific pathogens through the presence of dyes, but typically require specialized microscopes and narrow-band optical filters to extract information. We present a novel method of using polarizers in cross orientation with each other to filter out excitation light and allow detection of low signal levels of fluorescence with a simple intensity-based detector in the presence of high levels of excitation light. This concept is demonstrated using an inverted microscope fitted with a halogen lamp as the excitation source and an organic photovoltaic (PV) cell as the intensity detector. The excitation light is linearly polarized and used to illuminate a microfluidic device containing a 50µl volume of Rhodamine 6G dye dissolved in water. The detector (with a second polarizer orientated perpendicularly to the first) is placed over the microfluidic device. The resulting emission signal was detected by the organic PV cell down to a concentration of 100 nM This suggests that an integrated microfluidic device, with a PV detector and an organic light emitting excitation source and integrated polarizers, could be fabricated to realize a economical "lab on a chip" device for fluorescence assays.
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Andrea Pais, Andrea Pais, Haichuan Mu, Haichuan Mu, Erik Peterson, Erik Peterson, Ansuman Banerjee, Ansuman Banerjee, Ian Papautsky, Ian Papautsky, David Klotzkin, David Klotzkin, "Cross-polarization scheme for fluorescence detection for biochip and biomedical applications", Proc. SPIE 6465, Microfluidics, BioMEMS, and Medical Microsystems V, 64650M (22 January 2007); doi: 10.1117/12.720741; https://doi.org/10.1117/12.720741
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