1 May 2007 Data and image processing for a simultaneous time-and spectrum-resolved multifocal multiphoton microscopy
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Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65341S (2007) https://doi.org/10.1117/12.741586
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China
Abstract
A Simultaneous Time- and Spectrum-Resolved Multifocal Multiphoton Microscopy (STSR-MMM) system has been developed for the acquisition of fluorescence lifetime information of any spectrum of the biological samples in only one measurement. The near-infrared light from mode-locked titanium: sapphire femtosecond laser is split with a microlens array into an array of beams that are transformed into an array of high-aperture foci at the sample for parallel multiphoton excitation. The Gaussian shape of the excitation beam results in non-uniformity and asymmetry of the excitation foci array and also of the reconstructed fluorescence image. A coefficient matrix has been presented for the correction of the non-uniformity and asymmetry in the fluorescence image. Data acquisition and image processing methods have also been investigated to increase the image acquisition speed and improve the reconstruction of time- and spectrum-resolved fluorescence image. The methods for obtaining fluorescence intensity image, spectrally resolved intensity image and spectrally resolved lifetime image are also presented.
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Zhe Fu, Zhe Fu, Junle Qu, Junle Qu, Ziyang Lin, Ziyang Lin, Lixin Liu, Lixin Liu, Baoping Guo, Baoping Guo, Hanben Niu, Hanben Niu, } "Data and image processing for a simultaneous time-and spectrum-resolved multifocal multiphoton microscopy", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341S (1 May 2007); doi: 10.1117/12.741586; https://doi.org/10.1117/12.741586
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