Study the autofluorescence of microalgae inducted by laser using laser scanning confocal microscope

Lin Ou; Hui-ru Zhuang; Rong Chen; Jin-pin Lei; Xiang-ying Huang; Yan Tian; Shen Lin; Li-jiang Wang

doi:10.1117/12.741587

1 May 2007 Study the autofluorescence of microalgae inducted by laser using laser scanning confocal microscope

Lin Ou, Hui-ru Zhuang, Rong Chen, Jin-pin Lei, Xiang-ying Huang, Yan Tian, Shen Lin, Li-jiang Wang

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65341T (2007) https://doi.org/10.1117/12.741587
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

In this present paper, the microalgae irradiated by Nd:YAP laser are observed using Laser Confocal Scan microscope, and the auto fluorescence of microalgae cells is analyzed with quantitative analysis system, so as to investigate the effect of auto fluorescence alteration on growth of irradiated microalgae. Method: Platymonas subcordiformis, Phaeodactylum tricormutum and Isochyrsis zhanjiangensis are irradiated by Nd:YAP laser with 10w at 1341nm, irradiating time:12s, 30s, 35s and 55s. On Lambda Scan mode, the auto fluorescence images and fluorescence spectra of algae cells are obtained by the excitation 488nm (Ar⁺ laser). Result: there are two summit peaks, 489.8nm and 680.9nm, for Platymonas subcordiformis, Phaeodactylum tricormutum and Isochyrsis zhanjiangensis fluorescence spectrum. Contrast to the CK, the peak positions of fluorescence of irradiated algae cells is similar to the whole while the peak light intensity alters. On irradiation of promoting dose, however, the auto fluorescence intensity is enhanced more than control, with different type of microalgae, the different fluorescence intensity are shown in the analysis, and all of the microalgae pigment bodies change can be seen also. Conclusion: Physiological incentive effect of irradiated microalgae can be given expression on fluorescence characteristics and fluorescence intensity alteration of cells.

Citation Download Citation

Lin Ou, Hui-ru Zhuang, Rong Chen, Jin-pin Lei, Xiang-ying Huang, Yan Tian, Shen Lin, and Li-jiang Wang "Study the autofluorescence of microalgae inducted by laser using laser scanning confocal microscope", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341T (1 May 2007); https://doi.org/10.1117/12.741587

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KEYWORDS

Luminescence

Confocal microscopy

Microscopes

Laser scanners

Argon

Laser applications

Oceanography

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