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1 May 2007 Influencing factors in the measurement of intracellular free calcium
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Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65342E (2007) https://doi.org/10.1117/12.741298
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China
Abstract
One of the popular methods for measurement of intracellular free calcium ([Ca2+]i) is ratiometric fluorescence technique. Rmax and Rmin play an important role in the calibration of [Ca2+]i measurement. The purpose of this study was to analyze influencing factors in the [Ca2+]i measurement. Rmax and Rmin deduced from theory had a hyperbolic relation with the ratio of Cf and Cb (Cf: free indicator concentration; Cb: Ca2+-bound indicator concentration). In experiments, using radiometric fluorescence imaging system, they were measured separately in two types of cell lines in concentration gradient (2×105cells/ml, 5×105cells/ml, 1×106cells/ml and 2×106cells/ml). By Triton method, Rmax and Rmin of K562 cells were 1.21 and 0.20, respectively, and that of Hepa1-6 cells were 2.02 and 1. These results indicated that Rmax and Rmin were not influenced by concentration gradient in a certain range of cell concentration, but they were different in various types of cell lines.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yuanyuan Li, Sujuan Zhang, Dan Sun, Ying Qu, and Xinliang Zheng "Influencing factors in the measurement of intracellular free calcium", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65342E (1 May 2007); https://doi.org/10.1117/12.741298
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