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We report on the first application of silica-gold nanoshells to a solid-phase dot immunoassay. The assay principle is based
on staining of a drop (1μL ) analyte on a nitrocellulose membrane strip by using silica/gold nanoshells conjugated with
biospecific probing molecules. Experimental example is human IgG (hIgG, target molecules) and protein A (probing
molecules). For usual 15-nm colloidal gold conjugates, the minimal detectable amount of hIgG is about 4ng. By contrast,
for nanoshell conjugates (silica core diameter of 70 nm and gold outer diameter of 100 nm) we have found significant
increase in detection sensitivity and the minimal detectable amount of hIgG is about 0.5 ng.
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Boris N. Khlebtsov, Lev A. Dykman, Vadimir A. Bogatyrev, Nikolai G. Khlebtsov, "Gold nanoshells as solid-phase dot assay labels," Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65343L (1 May 2007); https://doi.org/10.1117/12.741502