In vivo flow cytometry and time-resolved near-IR angiography and lymphography

Ekaterina I. Galanzha M.D.; Valery V. Tuchin; Robert W. Brock; Vladimir P. Zharov

doi:10.1117/12.741087

27 April 2007 In vivo flow cytometry and time-resolved near-IR angiography and lymphography

Ekaterina I. Galanzha M.D., Valery V. Tuchin, Robert W. Brock, Vladimir P. Zharov

Proceedings Volume 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII; 65350K (2007) https://doi.org/10.1117/12.741087
Event: Saratov Fall Meeting 2006, 2006, Saratov, Russian Federation

Abstract

Integration of photoacoustic and photothermal techniques with high-speed, high-resolution transmission and fluorescence microscopy shows great potential for in vivo flow cytometry and indocyanine green (ICG) near-infrared (IR) angiography of blood and lymph microvessels. In particular, the capabilities of in vivo flow cytometry using rat mesentery and nude mouse ear models are demonstrated for real-time quantitative detection of circulating and migrating individual blood and cancer cells in skin, mesentery, lymph nodes, liver, kidney; studying vascular dynamics with a focus on lymphatics; monitoring cell traffic between blood and lymph systems; high-speed imaging of cell deformability in flow; and label-free real-time monitoring of single cell extravasation from blood vessel lumen into tissue. As presented, the advantages of ICG IR-angiography include estimation of time resolved dye dynamics (appearance and clearance) in blood and lymph microvessels using fluorescent and photoacoustic modules of the integrated technique. These new approaches are important for monitoring and quantifying metastatic and apoptotic cells; comparative measurements of plasma and cell velocities; analysis of immune responses; monitoring of circulating macromolecules, chylomicrons, bacteria, viruses and nanoparticles; molecular imaging. In the future, we believe that the integrated technique presented will have great potential for translation to early disease diagnoses (e.g. cancer) or assessment of innovative therapeutic interventions in humans.

Citation Download Citation

Ekaterina I. Galanzha M.D., Valery V. Tuchin, Robert W. Brock, and Vladimir P. Zharov "In vivo flow cytometry and time-resolved near-IR angiography and lymphography", Proc. SPIE 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII, 65350K (27 April 2007); https://doi.org/10.1117/12.741087

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