A high-resolution optical imaging system for obtaining the serial transverse section images of biologic tissue

Li Wu; Bin Zhang; Ping Wu; Qian Liu; Hui Gong

doi:10.1117/12.740969

26 April 2007 A high-resolution optical imaging system for obtaining the serial transverse section images of biologic tissue

Li Wu, Bin Zhang, Ping Wu, Qian Liu, Hui Gong

Author Affiliations +

Proceedings Volume 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII; 65350Z (2007) https://doi.org/10.1117/12.740969
Event: Saratov Fall Meeting 2006, 2006, Saratov, Russian Federation

Abstract

A high-resolution optical imaging system was designed and developed to obtain the serial transverse section images of the biologic tissue, such as the mouse brain, in which new knife-edge imaging technology, high-speed and high-sensitive line-scan CCD and linear air bearing stages were adopted and incorporated with an OLYMPUS microscope. The section images on the tip of the knife-edge were synchronously captured by the reflection imaging in the microscope while cutting the biologic tissue. The biologic tissue can be sectioned at interval of 250 nm with the same resolution of the transverse section images obtained in x and y plane. And the cutting job can be automatically finished based on the control program wrote specially in advance, so we save the mass labor of the registration of the vast images data. In addition, by using this system a larger sample can be cut than conventional ultramicrotome so as to avoid the loss of the tissue structure information because of splitting the tissue sample to meet the size request of the ultramicrotome.

Citation Download Citation

Li Wu, Bin Zhang, Ping Wu, Qian Liu, and Hui Gong "A high-resolution optical imaging system for obtaining the serial transverse section images of biologic tissue", Proc. SPIE 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII, 65350Z (26 April 2007); https://doi.org/10.1117/12.740969

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KEYWORDS

Tissues

Imaging systems

Microscopes

Optical imaging

Optical resolution

Tissue optics

Brain

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