27 April 2007 Development of SPME-HPLC methodology for detection of nitroexplosives
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Abstract
Solid phase microextraction (SPME) has been coupled with liquid chromatography to widen its range of application to nonvolatile and thermally unstable compounds, generally limited for SPME-GC. A method for analysis of nitroaromatic explosives and its degradations products was developed by coupling SPME and high performance liquid chromatography with ultraviolet detection (HPLC/UV), introducing a modified interface that ensure accuracy, precision, repeatability, high efficiency, unique selectivity and high sensitive to detection and quantification of explosives from surface soil samples and increased chromatographic efficiency. A pretreatment step was introduced for the soil samples which extracted the target compounds into an aqueous phase. Several parameters that affect the microextraction were evaluated, such as: fiber coating, adsorption and desorption time and stirring rate. The effect of salting out (NaCl) on analyte extraction and the role of various solvents on SPME fiber were also evaluated. Carbowax-templated resin (CW/TPR) and Polydimethilsiloxane-divinilbenzene (PDMS-DVB) fibers were used to extract the analytes from the aqueous samples. Explosives were detected at low &mgr;g/mL concentrations. This study demonstrates that SPME-HPLC is a very promising method of analysis of explosives from aqueous samples and has been successfully applied to the determination of nitroaromatic compounds, such as TNT.
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Sandra L. Peña-Luengas, Sandra L. Peña-Luengas, Jackeline I Jerez-Rozo, Jackeline I Jerez-Rozo, Sandra N Correa, Sandra N Correa, Nelson E. Peña, Nelson E. Peña, Samuel P. Hernández-Rivera, Samuel P. Hernández-Rivera, "Development of SPME-HPLC methodology for detection of nitroexplosives", Proc. SPIE 6553, Detection and Remediation Technologies for Mines and Minelike Targets XII, 65531W (27 April 2007); doi: 10.1117/12.720362; https://doi.org/10.1117/12.720362
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