26 April 2007 Developments in on-the-fly biomarking: a new method to rapidly identify chemical and biological aerosols
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Abstract
We report on the advances made in the basic research to label specific chemical or biological aerosols on-the-fly using an electrospray technique. Fluorescent biomarkers that have been created for specific targets, and that produce a detectable change in emission characteristics only upon binding, will be used to coat all aerosols in an air stream. Aerosols with appropriate receptors will be labeled in this manner, allowing them to be identified in near real-time using a simple laser-induced fluorescence technique. In effect, an immunoassay is quickly performed on the surface of single chemical or biological particles as they flow in an air stream, labeling specific ones for rapid, single-particle interrogation and identification among a diverse and dynamic background. This method permits the use of solutions containing mixtures of different biomarkers to simultaneously identify multiple types of chemical or biological aerosols. Some issues that are currently being investigated include the kinetics of biomarker surface binding to an aerosol in flight and the control of charged aerosols for efficient single particle interrogation.
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Matthew B. Hart, Matthew B. Hart, Horn-Bond Lin, Horn-Bond Lin, Jason Deich, Jason Deich, Charles D. Merritt, Charles D. Merritt, Jay D. Eversole, Jay D. Eversole, } "Developments in on-the-fly biomarking: a new method to rapidly identify chemical and biological aerosols", Proc. SPIE 6554, Chemical and Biological Sensing VIII, 65540P (26 April 2007); doi: 10.1117/12.719745; https://doi.org/10.1117/12.719745
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