27 April 2007 Investigation of synthetic molecular recognition for biosensing applications
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A fundamental understanding of the factors which influence binding performance is critical to any technology or methodology relying on molecular recognition of a specific target species. For the Army, there is a growing need for a basic understanding of these interactions with traditional recognition elements (e.g., antibodies) in non-traditional environmental conditions, such as with new and emerging threats. There is a similar need for building a base of knowledge on non-traditional affinity ligands that are biomimetic or biosynthetic in nature. In this paper, specific research at the Army Research Laboratory towards the development, evaluation and use of synthetic affinity ligands for sensing applications is discussed. This includes the results of our investigations of aptamer-based affinity ligands targeting Campylobacter jejuni. Using capillary electrophoretic techniques, the relative binding affinities of the aptamer ligands towards the target pathogen as well as the degree of cross-reactivity with other food borne-pathogens (i.e., Escherichia coli O157:H7 and Salmonella typhimurium) were evaluated. Current progress towards the development of synthetic affinity ligands for sensing applications will also be discussed.
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Dimitra N. Stratis-Cullum, Sun McMasters, Letha J. Sooter, and Paul M. Pellegrino "Investigation of synthetic molecular recognition for biosensing applications", Proc. SPIE 6554, Chemical and Biological Sensing VIII, 65540Z (27 April 2007); doi: 10.1117/12.722432; https://doi.org/10.1117/12.722432

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