The influence of the protoporphyrin diamino acid derivatives photodynamic effect on the activity of selected enzymes

Anna Romiszewska; Dariusz Pietrzykowski; Alfreda Graczyk

doi:10.1117/12.726525

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23 January 2007 The influence of the protoporphyrin diamino acid derivatives photodynamic effect on the activity of selected enzymes

Anna Romiszewska, Dariusz Pietrzykowski, Alfreda Graczyk

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Proceedings Volume 6598, Laser Technology VIII: Applications of Lasers; 659803 (2007) https://doi.org/10.1117/12.726525
Event: Laser Technology VIII, 2006, Szczecin, Poland

Abstract

Photodynamic Therapy (PDT) requires three components: a dye applied as the photosensitizer, a source of light, and a possibly large amount of oxygen dissolved in the tissue. The considerably higher absorption of porphyrin photosensitizers by tumorous tissues allows the application of these photosensitizers in the diagnosis and therapy of tumors. The cytotoxic and cytolethal effects of the photodynamic therapy are associated with the formation of a singlet oxygen or production of radical species by the porphyrin photosensitizers. In the cell, the singlet oxygen or radical species impair important macromolecules like membrane phospholipids, structural proteins, enzymes, and DNA. This study describes the photodynamic effect of the PP(AA)₂Arg₂ group photosensitizers on the activity of adenosine deaminase, DNA-ase and trypsin in relation to the photosensitizer dose and the excitation energy.

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Anna Romiszewska, Dariusz Pietrzykowski, and Alfreda Graczyk "The influence of the protoporphyrin diamino acid derivatives photodynamic effect on the activity of selected enzymes", Proc. SPIE 6598, Laser Technology VIII: Applications of Lasers, 659803 (23 January 2007); https://doi.org/10.1117/12.726525

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