Liposome characterization with fluorescence cumulant analysis

Joseph E. Reiner; Andreas Jahn; Laurie E. Locascio; Michael Gaitan; John J. Kasianowicz

doi:10.1117/12.726803

8 June 2007 Liposome characterization with fluorescence cumulant analysis

Joseph E. Reiner, Andreas Jahn, Laurie E. Locascio, Michael Gaitan, John J. Kasianowicz

Proceedings Volume 6602, Noise and Fluctuations in Biological, Biophysical, and Biomedical Systems; 66020I (2007) https://doi.org/10.1117/12.726803
Event: SPIE Fourth International Symposium on Fluctuations and Noise, 2007, Florence, Italy

Abstract

Liposomes are self-assembled spherical vesicles comprised of a lipid bilayer membrane that segregates an internal aqueous environment from an external aqueous environment. These nanometer-scale structures have demonstrated potential for targeted drug delivery applications. For liposomes to be useful in vivo, the liposome size and dosage of molecules contained within them needs to be controlled. We present here a fluorescence-based technique for characterizing the relative encapsulation efficiency, leakage rate, and shelf life of liposome formulations. We report results from three different liposome solutions over a period of two months that show the liposome brightness remains stable while the background dye concentration increases. These parameters may prove useful for optimizing the liposome formation process.

Citation Download Citation

Joseph E. Reiner, Andreas Jahn, Laurie E. Locascio, Michael Gaitan, and John J. Kasianowicz "Liposome characterization with fluorescence cumulant analysis", Proc. SPIE 6602, Noise and Fluctuations in Biological, Biophysical, and Biomedical Systems, 66020I (8 June 2007); https://doi.org/10.1117/12.726803

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