Translator Disclaimer
5 March 2007 Phthalocyanine-assisted photodynamic inactivation of pathogenic microorganisms
Author Affiliations +
Proceedings Volume 6604, 14th International School on Quantum Electronics: Laser Physics and Applications; 66042A (2007) https://doi.org/10.1117/12.727717
Event: 14th International School on Quantum Electronics: Laser Physics and Applications, 2006, Sunny Beach, Bulgaria
Abstract
The phthalocyanine zinc(II) and aluminum (III) complexes were studied to photoinactivate the bacterial strains, Staphylococcus aureus, methacillin-sensitive and methacillin-resistant, Pseudomonas aeruginosa and one yeast Candida albicans. The binding of phthalocyanines to bacteria and fungi cells was evaluated by the means of laserinduced fluorescence technique. The fluorescent spectra of dyes (650 - 800 nm) after direct excitation (635 nm) were measured as follows: 1. for the aqua supernatants obtained after 10 min cell incubation with the respected phthalocyanines (1.6 &mgr;mol.l-1), 2. for the washed from the unbound dye cells, and 3. for the organic extracts from the three times washed cells. Fluorescent intensities at the emission maximum (~690 nm) were compared to the spectra of the phthalocyanines in organic solutions. The phthalocyanines uptake data for bacteria and fungi were determined at different cell densities. Nevertheless the better fluorescence properties of AlPc (fluorescent quantum yield of 0.4 towards 0.3 for ZnPcs) the lower drug accumulation in microorganisms was obtained. PDI results indicated an intensive lowering of the bacterial survival of both strains of S. aureus treated with cationic ZnPcMe followed by the anionic ZnPcS, at irradiance of 100 mW cm-2 and fluence rate of 60 J cm-2. More resistant to phototreatment P. aeruginosa and morphologically complicated yeast C. albicans were successfully inactivated only with cationic ZnPcMe. These data indicate the promising future application of cationic phthalocyanine in photodynamic inactivation of pathogenic microorganisms.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Vanya Mantareva, Ivan Angelov, Ekaterina Borissova, Latchezar Avramov, and Vesselin Kussovski "Phthalocyanine-assisted photodynamic inactivation of pathogenic microorganisms", Proc. SPIE 6604, 14th International School on Quantum Electronics: Laser Physics and Applications, 66042A (5 March 2007); https://doi.org/10.1117/12.727717
PROCEEDINGS
5 PAGES


SHARE
Advertisement
Advertisement
Back to Top