25 April 2007 2D and 3D printing of biomolecules employing femtosecond lasers
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Proceedings Volume 6606, Advanced Laser Technologies 2006; 66061F (2007) https://doi.org/10.1117/12.730199
Event: Advanced Laser Technologies 2006, 2006, Brasov, Romania
Abstract
We demonstrate two- and three-dimensional patterning of biological molecules. For the two-dimensional patterning we employ Laser-Induced Forward Transfer of materials in solution. For the three-dimensional patterning we employ femtosecond-laser induced three-photon polymerization, a technique which enables the construction of arbitrary 2D and 3D structures of submicron resolution. Biotin is subsequently attached to the 3D structures via UV-activated crosslinking. The integrity of the photolytically immobilized biotin is confirmed by detecting the binding of fluorescently labeled avidin via fluorescence microscopy.
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Maria Farsari, V. Dinca, M. Dinescu, T. S. Drakakis, C. Fotakis, "2D and 3D printing of biomolecules employing femtosecond lasers", Proc. SPIE 6606, Advanced Laser Technologies 2006, 66061F (25 April 2007); doi: 10.1117/12.730199; https://doi.org/10.1117/12.730199
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