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11 July 2007 Sensing metabolic activity in tissue engineered constructs
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Tissue engineered constructs can be employed to graft wounds or replace diseased tissue. Non-invasive methods are required to assess cellular viability in these constructs both pre- and post-implantation into patients. In this study, Monte Carlo simulations and fluorescence experiments were executed on ex vivo produced oral mucosa equivalent (EVPOME) constructs to investigate the fluorescence emitted at 355 nm excitation from these constructs. Both simulations and experiments indicated the need to investigate alternative excitation wavelengths in order to increase the cellular fluorescence from these constructs, while decreasing contributions from extra-cellular fluorophores.
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Malavika Chandra, Robert H. Wilson, Wen-Liang Lo, Karthik Vishwanath, Kenji Izumi, Stephen E. Feinberg, and Mary-Ann Mycek "Sensing metabolic activity in tissue engineered constructs", Proc. SPIE 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, 66280B (11 July 2007); doi: 10.1117/12.729426;

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