Study of antiangiogenic drugs by fluorescence imaging and spectroscopy of a contrast agent in mice

G. Valentini; C. D'Andrea; R. Ferrari; A. Pifferi; R. Cubeddu; D. Caronia; M. Martinelli; R. Giavazzi

doi:10.1117/12.728193

19 July 2007 Study of anti-angiogenic drugs by fluorescence imaging and spectroscopy of a contrast agent in mice

G. Valentini, C. D'Andrea, R. Ferrari, A. Pifferi, R. Cubeddu, D. Caronia, M. Martinelli, R. Giavazzi

Proceedings Volume 6628, Diagnostic Optical Spectroscopy in Biomedicine IV; 66280P (2007) https://doi.org/10.1117/12.728193
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

We used two fluorescence techniques based on the Indocyanine Green contrast agent to study the effectiveness of antiangionenic drugs in mice. To this purpose, the volume of the active vasculature in different tumor models implanted in mice was assessed by means of a low noise fluorescence imaging setup and by a photon counting system working in transmittance geometry. Using a first tumor model (carcinoma MDA-MB-435) we observed that mice treated with a Vascular Disrupting Agent (ZD6126) showed a reduction in fluorescence emission of the contrast agent with respect to control mice. This was a clear indication of the vascular shutdown that took place in tumors. The effectiveness of the treatment was also confirmed by histological sections. Then, in a second experiment we considered a second tumor model (carcinoma 1A9-VS1) overexpressing the Vascular Endotelial Growth Factor (VEGF121), which is used by tumor cells to promote angiogenesis. We measured the Indocyanine Green fluorescence in mice treated with an antioangiogenic drug (Avastin^TM) and in control mice. In tumors of treated mice we observed an ICG emission lower than the one detected in control mice. This demonstrated that VEGF activity was effectively blocked by the treatment with Avastin. In conclusion, ICG fluorescence provides a simple and reliable way to assess the effectiveness of vascular targeting therapies. Measurements of the fluorescence signal can be repeated every 24 hours, thus allowing oncologists to perform longitudinal studies on the same animals.

Citation Download Citation

G. Valentini, C. D'Andrea, R. Ferrari, A. Pifferi, R. Cubeddu, D. Caronia, M. Martinelli, and R. Giavazzi "Study of anti-angiogenic drugs by fluorescence imaging and spectroscopy of a contrast agent in mice", Proc. SPIE 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, 66280P (19 July 2007); https://doi.org/10.1117/12.728193

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Tumors

Imaging spectroscopy

Tissues

Transmittance

Tumor growth modeling

Fluorescence spectroscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years