The present work aims a new medical probe for surgeons devoted to brain cancers, in particular
glioblastoma multiforme. Within the last years, our group has started the development of a new intra-operative
beta imaging probe. More recently, we took an alternative approach for the same application: a
fluorescence probe. In both cases the purpose is to differentiate normal from tumor brain tissue.
In a first step, we developed set-ups capable to measure autofluorescence. They are based on a
dedicated epi-fluorescence design and on specific fiber optic probes. Relative signal amplitude, spectral
shape and fluorescence lifetime measurements are foreseen to distinguish normal and cancer tissue by
analyzing fluorophores like NADH, lipopigments and porphyrines. The autofluorescence spectra are
recorded in the 460-640 nm range with a low resolution spectrometer. For lifetime measurements a fast
detector (APD) is used together with a TCSPC-carte. Intrinsic wavelength- and time-resolutions are a few
nm and 200 ps, respectively. Different samples have been analyzed to validate our new detection system
and to allow a first configuration of our medical fluorescence probe. First results from the tissue
measurements are shown.
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