20 July 2007 Utilizing nonlinear optical microscopy to investigate the development of early cancer in nude mice in vivo
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Abstract
In this investigation, we used in vivo nonlinear optical microscopy to image normal and carcinogen DMBA treated skin tissues of nude mice. We acquired two-photon autofluroescence and second harmonic generation (SHG) images of the skin tissue, and applied the ASI (Autofluorescence versus SHG Index) to the resulting image. This allows us to visualize and quantify the interaction between mouse skin cells and the surrounding connective tissue. We found that as the imaging depth increases, ASI has a different distribution in the normal and the treated skin tissues. Since the DMBA treated skin eventually became squamous cell carcinoma (SCC), our results show that the physiological changes to mouse skin en route to become cancer can be effectively tracked by multiphoton microscopy. We envision this approach to be effective in studying tumor biology and tumor treatment procedures.
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Chun-Chin Wang, Feng-Chieh Li, Sung-Jan Lin, Wen Lo, Chen-Yuan Dong, "Utilizing nonlinear optical microscopy to investigate the development of early cancer in nude mice in vivo", Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300Y (20 July 2007); doi: 10.1117/12.728066; https://doi.org/10.1117/12.728066
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KEYWORDS
Skin

Second-harmonic generation

Cancer

Tissues

In vivo imaging

Microscopy

Collagen

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