Improvement of axial resolution in confocal microscopy using heterodyne illumination

SeungWoo Lee; Hongki Yoo; Byung Seon Chun; Wanhee Chun; Dae-gab Gweon

doi:10.1117/12.727936

8 August 2007 Improvement of axial resolution in confocal microscopy using heterodyne illumination

SeungWoo Lee, Hongki Yoo, Byung Seon Chun, Wanhee Chun, Dae-gab Gweon

Proceedings Volume 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III; 663012 (2007) https://doi.org/10.1117/12.727936
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

A new technique for improving the axial resolution of confocal microscope is proposed. Based on the interference between two different frequency beams, which are separated axially, a frequency domain field confined focal spot is generated. The effective region made by the interference makes the point-spread function (PSF) of confocal microscope sharper. The three-dimensional imaging equations are derived. The intensity distribution of frequency domain field confined focal spot is proportional to the absolute value of the product of two fields. Three-dimensional intensity pointspread function (IPSF) is calculated numerically. The farther two beams are separated axially, the sharper IPSF is obtained. The numerical results show that the full width half maximum (FWHM) of the IPSF is improved by factor of 1.78 maintaining the strength of side lobe at 0.5 relative to main lobe. Also simulations for two-point resolution show the same improvement in the axial resolution.

Citation Download Citation

SeungWoo Lee, Hongki Yoo, Byung Seon Chun, Wanhee Chun, and Dae-gab Gweon "Improvement of axial resolution in confocal microscopy using heterodyne illumination", Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 663012 (8 August 2007); https://doi.org/10.1117/12.727936

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