13 July 2007 Physical limits to autofluorescence signals in vivo recordings in the rat olfactory bulb: a Monte Carlo study
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Understanding the cellular mechanisms of energy supply to neurons following physiological activation is still challenging and has strong implications to the interpretation of clinical functional images based on metabolic signals such as Blood Oxygen Level Dependent Magnetic Resonance Imaging or 18F-Fluorodexoy-Glucose Positron Emission Tomography. Intrinsic Optical Signal Imaging provides with high spatio temporal resolution in vivo imaging in the anaesthetized rat. In that context, intrinsic signals are mainly related to changes in the optical absorption of haemoglobin depending on its oxygenation state. This technique has been validated for imaging of the rat olfactory bulb, providing with maps of the actived olfactory glomeruli, the functional modules involved in the first step of olfactory coding. A complementary approach would be autofluorescence imaging relying on the fluorescence properties of endogenous Flavin Adenine Dinucleotide (FAD) or Nicotinamide Adenine Dinucleotide (NADH) both involved in intracellular metabolic pathways. The purpose of the present study was to investigate the feasibility of in vivo autofluorescence imaging in the rat olfactory bulb. We performed standard Monte Carlo simulations of photons scattering and absorption at the excitation and emission wavelengths of FAD and NADH fluorescence. Characterization of the fluorescence distribution in the glomerulus, effect of hemoglobin absorption at the excitation and absorption wavelengths as well as the effect of the blurring due to photon scattering and the depth of focus of the optical apparatus have been studied. Finally, optimal experimental parameters are proposed to achieve in vivo validation of the technique in the rat olfactory bulb.
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B. L'Heureux, B. L'Heureux, H. Gurden, H. Gurden, L. Pinot, L. Pinot, R. Mastrippolito, R. Mastrippolito, F. Lefebvre, F. Lefebvre, P. Lanièce, P. Lanièce, F. Pain, F. Pain, } "Physical limits to autofluorescence signals in vivo recordings in the rat olfactory bulb: a Monte Carlo study", Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66331Y (13 July 2007); doi: 10.1117/12.728209; https://doi.org/10.1117/12.728209

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