Preparative separations using optical chromatography

Alexander Terray; Jonathan Arnold; Steven D. Sundbeck; Tomasz A. Leski; Sean J. Hart

doi:10.1117/12.742080

5 September 2007 Preparative separations using optical chromatography

Alexander Terray, Jonathan Arnold, Steven D. Sundbeck, Tomasz A. Leski, Sean J. Hart

Proceedings Volume 6644, Optical Trapping and Optical Micromanipulation IV; 66441U (2007) https://doi.org/10.1117/12.742080
Event: NanoScience + Engineering, 2007, San Diego, California, United States

Abstract

Optical Chromatography, used for particle separation, involves loosely focusing a laser into a fluid flowing opposite the direction of laser propagation. When microscopic particles in the flow path encounter this beam they are trapped axially along the beam and are pushed upstream from the laser focal point to rest at a point where the optical and fluid forces on the particle balance. Because optical and fluid forces are sensitive to differences in the physical and chemical properties of a particle, fine separations are possible. An optical chromatography beam which completely fills a fluid channel can operate as an optically tunable filter for the separation of polymeric/colloidal and biological samples. We will show how the technique can be used to separate injected samples containing large numbers of colloids. The power of optical chromatographic separations will be illustrated through the combination with other analytical techniques.

Citation Download Citation

Alexander Terray, Jonathan Arnold, Steven D. Sundbeck, Tomasz A. Leski, and Sean J. Hart "Preparative separations using optical chromatography", Proc. SPIE 6644, Optical Trapping and Optical Micromanipulation IV, 66441U (5 September 2007); https://doi.org/10.1117/12.742080

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