Detection method and experimental study for pollutant content based on surface plasmon resonance biosensor

Guangcan Zhao; Xiaoping Wang; Xuejiao Zhang

doi:10.1117/12.783326

27 November 2007 Detection method and experimental study for pollutant content based on surface plasmon resonance biosensor

Guangcan Zhao, Xiaoping Wang, Xuejiao Zhang

Author Affiliations +

Proceedings Volume 6723, 3rd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment; 67232Q (2007) https://doi.org/10.1117/12.783326
Event: 3rd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Large Mirrors and Telescopes, 2007, Chengdu, China

Abstract

The principle of SPR biosensor is presented at the beginning of this paper. Then both direct assay for macromolecules and indirect assay (inhibition assay) for small molecules are introduced, which allow for high sensitive SPR detection of these two kinds of molecules. By combining some peripheral components including flow cell, multiposition valve, constant flowrate syringe pump and so on, a SPR biosensor system with an integrated miniature SPR sensor was designed and produced. Solutions of different concentrations of bovine serum albumin (BSA) were detected by making use of this system with the method of direct assay and a sensorgram of SPR resonance angle about different concentrations of BSA antibodies was generated. A calibration curve of BSA antibodies was also obtained. Solutions of different concentrations of estradiols were detected using inhibition assay. According to SPR resonance angle shift (ΔRU) corresponding to different concentrations of estradiols, a calibration curve was generated and the result was satisfying. For the sample of unknown concentration, once the resonance angle shift (ΔRU) is detected, the concentration can be calculated from the calibration curve.

Citation Download Citation

Guangcan Zhao, Xiaoping Wang, and Xuejiao Zhang "Detection method and experimental study for pollutant content based on surface plasmon resonance biosensor", Proc. SPIE 6723, 3rd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment, 67232Q (27 November 2007); https://doi.org/10.1117/12.783326

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