27 July 2007 New perspectives in protein-based biosensors: the glucokinase from B. stearothermophilus and the odorant-binding protein from C. familiaris as probes for non-consuming analyte sensors
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Abstract
Glucose sensing and odorant molecules sensing are used as a models to explore the advantages and problems deriving from the use of either enzymes or odorant-binding proteins to develop stable optical biosensors. We report on a novel approach to address the problem of substrate consumption by sensors based on enzymes, namely the utilization of apoenzymes as non-active forms of the protein which are still able to bind the substrate/ligand. We also show studies in which the isolation of an odorant-binding protein from the nose of the dog is used as non-consuming analyte probe for the realization of an integrated optical sensor.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Irina M. Kuznetsova, Irina M. Kuznetsova, Olga Povarova, Olga Povarova, Olesya Stepanenko, Olesya Stepanenko, Konstantin K. Turoverov, Konstantin K. Turoverov, Roberta Crescenzo, Roberta Crescenzo, Antonio Varriale, Antonio Varriale, Maria Staiano, Maria Staiano, Sabato D'Auria, Sabato D'Auria, } "New perspectives in protein-based biosensors: the glucokinase from B. stearothermophilus and the odorant-binding protein from C. familiaris as probes for non-consuming analyte sensors", Proc. SPIE 6733, International Conference on Lasers, Applications, and Technologies 2007: Environmental Monitoring and Ecological Applications; Optical Sensors in Biological, Chemical, and Engineering Technologies; and Femtosecond Laser Pulse Filamentation, 673318 (27 July 2007); doi: 10.1117/12.753249; https://doi.org/10.1117/12.753249
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