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7 August 2007 Development of human granulocyte morphometric model for scanning flow cytometry
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Proceedings Volume 6734, International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine; 67341C (2007) https://doi.org/10.1117/12.753259
Event: International Conference on Lasers, Applications, and Technologies '07, 2007, Minsk, Belarus
Abstract
Cell structure, shape, and size distribution are characterized by optical measurement of normal peripheral blood granulocytes in a cell suspension as applied to a cell discrimination by the solution of the direct and inverse light-scattering problems in scanning flow cytometry (SFC). The distribution laws of the cell and nuclei sizes are estimated. A morphometric model of a segmented neutrophilic granulocyte is constructed on the base of the gained data. An interrelation between the cell and nucleus metric characteristics is deduced. The obtained interrelation allows decreasing a number of independent parameters of the cell model to adapt it to the real-time discrimination of the cells. The findings can be used as input parameters for the solution of the direct and inverse light-scattering problems in SFC-dispensing with a costly and time-consuming immunophenotyping of the cells.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Gennady I. Ruban, Svetlana M. Kosmacheva, Natalia V. Goncharova, and Valery A. Loiko "Development of human granulocyte morphometric model for scanning flow cytometry", Proc. SPIE 6734, International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine, 67341C (7 August 2007); https://doi.org/10.1117/12.753259
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