25 September 2007 Tracking SERS-active nanoprobe intracellular uptake for chemical and biological sensing
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A critical aspect of the use of nanoprobes for intracellular studies in chemical and biological sensing involves a fundamental understanding of their uptake and trajectory in cells. In this study, we describe experiments using surface-enhanced Raman scattering (SERS) spectroscopy and mapping to track cellular uptake of plasmonics-active labeled nanoparticles. Three different Raman-active labels with positive, negative, and neutral charges were conjugated to silver colloidal nanoparticles with the aim of spatially and temporally profiling intracellular delivery and tracking of nanoprobes during uptake in single mammalian cells. 1-D Raman spectra and 2-D Raman mapping are used to identify and locate the probes via their SERS signal intensities. Because Raman spectroscopy is very specific for identification of chemical and molecular signatures, the development of functionalized plasmonics-active nanoprobes capable of exploring intracellular spaces and processes has the ability to provide specific information on the effects of biological and chemical pollutants in the intracellular environment. The results indicate that this technique will allow study of when, where, and how these substances affect cells and living organisms.
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Molly K. Gregas, Molly K. Gregas, Fei Yan, Fei Yan, Jonathan Scaffidi, Jonathan Scaffidi, Hsin-Neng Wang, Hsin-Neng Wang, Christopher Khoury, Christopher Khoury, Yan Zhang, Yan Zhang, Tuan Vo-Dinh, Tuan Vo-Dinh, } "Tracking SERS-active nanoprobe intracellular uptake for chemical and biological sensing", Proc. SPIE 6755, Advanced Environmental, Chemical, and Biological Sensing Technologies V, 67550H (25 September 2007); doi: 10.1117/12.758287; https://doi.org/10.1117/12.758287

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