4 October 2007 Optimization of oxygen sensitive optical dye membrane polymers for fluorescent-lifetime-based physiological biosensing
Author Affiliations +
Fiber optic based sensor technologies have many significant advantages over electrochemical sensors, and as a result have broad application for sensing in biology, agriculture and medicine. An important component of fiber optic biosensor is the sensing element. Usually, a polymer matrix containing the analyte specific fluorescent dye is immobilized on one end of the fiber optic probe. The polymer matrix provides mechanical stability to the immobilized membrane and entraps the fluorescent dye molecules. The target analyte diffuses into the membrane polymer and quenches the fluorescent dye. This optical interaction between the analyte and fluorescent dye dynamically changes the fluorescence lifetime of the dye. These changing fluorescent lifetimes reveal information about the target analyte concentration. Although the fluorescent lifetime of the dye is of primary interest, high signal-to-noise ratio (SNR) is also very desirable. Conventionally, complex electronics is implemented to achieve high SNR. Various signal processing methods such as signal amplification and filtering are implemented to achieve high SNR. In this study, we report optical signal amplification by modification of the dye-polymer matrix by addition of titanium oxide particles. The addition of titanium oxide particles enhanced the optical signal intensity. Optical performances of different sizes and concentrations of titanium oxide particles are compared. We believe that this increased optical intensity is due to increased optical scattering in the dye-polymer matrix. We also compare the performance of titanium oxide particles to gold and other material particles to experimentally probe the exact nature of light scattering in the dye-polymer matrix.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
M. R. Chatni, M. R. Chatni, D. E. Maier, D. E. Maier, D. M. Porterfield, D. M. Porterfield, } "Optimization of oxygen sensitive optical dye membrane polymers for fluorescent-lifetime-based physiological biosensing", Proc. SPIE 6759, Smart Biomedical and Physiological Sensor Technology V, 67590C (4 October 2007); doi: 10.1117/12.734252; https://doi.org/10.1117/12.734252

Back to Top