Determining two-photon absorption cross sections via nonresonant multiphoton photoacoustic spectroscopy

John B. Kiser; Nirmala Chandrasekharan; Brian M. Cullum

doi:10.1117/12.730458

5 October 2007 Determining two-photon absorption cross sections via nonresonant multiphoton photoacoustic spectroscopy

John B. Kiser, Nirmala Chandrasekharan, Brian M. Cullum

Proceedings Volume 6759, Smart Biomedical and Physiological Sensor Technology V; 67590L (2007) https://doi.org/10.1117/12.730458
Event: Optics East, 2007, Boston, MA, United States

Abstract

Multiphoton excitation of exogenous dyes and endogenous biochemical species has been used extensively for tissue diagnosis by fluorescence spectroscopy. Unfortunately, the majority of endogenous biochemical chromophores have low quantum yields, less than 0.2, therefore determining two-photon cross sections of weakly luminescencing molecules is difficult using two-photon fluorescence spectroscopy. Accurate determination of two-photon cross sections of these biochemicals could provide insight into fluorescence signal reduction caused by the absorption of excitation energy by non-target intracellular species. Non-resonant multiphoton photoacoustic spectroscopy (NMPPAS) is a novel technique we have developed for condensed matter measurements that has the potential for accurately determining two-photon absorption cross-sections of chemicals with small or non-existant fluorescence quantum yields. In this technique, near infrared light is used to generate an ultrasonic signal following a non-resonant two-photon excitation process. This ultrasonic wave is directly related to the non-radative relaxation of the chromophore of interest and is measured using a contact piezoelectric ultrasonic transducer. The signal from the ultrasonic transducer can then be used to calculate two-photon absorption cross sections. This paper will describe the validation of this technique by measuring the two-photon absorption cross- sections of well characterized chromophores such as rhodamine B and coumarin 1 in solution as well as riboflavin in a gelatin tissue phantom.

Citation Download Citation

John B. Kiser, Nirmala Chandrasekharan, and Brian M. Cullum "Determining two-photon absorption cross sections via nonresonant multiphoton photoacoustic spectroscopy", Proc. SPIE 6759, Smart Biomedical and Physiological Sensor Technology V, 67590L (5 October 2007); https://doi.org/10.1117/12.730458

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Absorption

Rhodamine B

Luminescence

Photoacoustic spectroscopy

Transducers

Tissues

Ultrasonics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years