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We present an intelligent microfluidic system with oxidase enzyme coupled biosensors. Baseline (zero-value) drift and
sensitivity degradation are two common problems related with biosensors. In order to overcome these problems there is a
great need for integrating an on-demand, in situ self-diagnosis and self-calibration unit along with the sensor. Utilizing
the microfluidic technology, we explore the feasibility of implementing this function without any externally coupled
bulky apparatus. A microsystem including a microfluidic channel and calibration electrodes are prepared by
microfabrication techniques. A novel method of using hydrogen and oxygen bubbles generated by electrolysis of water is
used to saturate the solution with these gases in the microfluidic channel where the biosensor is placed. The hydrogen
bubble provides oxygen-depleted microenvironment to conduct a zero-value calibration procedure for the sensor. The
oxygen bubble provides high sensitivity and constant oxygen background environment to allow stable enzyme reactions
that is not limited or perturbed by the fluctuation of background oxygen in sample solutions. Commercial oxygen
sensors and pH sensors are used to confirm whether saturation or depletion of oxygen has occurred with minimum local
pH change near the sensor during the electrolytic bubble generation. The glucose data obtained from the experiments
assure that our proposed method is promising to overcome the above mentioned two problems.
Nitin Radhakrishnan,Jongwon Park, andChang-Soo Kim
"Microfluidic biosensors for intelligent metabolite monitoring", Proc. SPIE 6759, Smart Biomedical and Physiological Sensor Technology V, 67590U (5 October 2007); https://doi.org/10.1117/12.737092
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Nitin Radhakrishnan, Jongwon Park, Chang-Soo Kim, "Microfluidic biosensors for intelligent metabolite monitoring," Proc. SPIE 6759, Smart Biomedical and Physiological Sensor Technology V, 67590U (5 October 2007); https://doi.org/10.1117/12.737092