Oxytetracycline analysis in honey using a specific portable analyzer

Guoying Chen; Daniel Schwartz; S. Braden; Alberto Nunez

doi:10.1117/12.735029

22 October 2007 Oxytetracycline analysis in honey using a specific portable analyzer

Guoying Chen, Daniel Schwartz, S. Braden, Alberto Nunez

Proceedings Volume 6761, Optics for Natural Resources, Agriculture, and Foods II; 67610K (2007) https://doi.org/10.1117/12.735029
Event: Optics East, 2007, Boston, MA, United States

Abstract

Oxytetracycline (OTC) residue in honey is detected using a portable analyzer designed to specifically target tetracycline (TC) drugs based on europium-sensitized luminescence (ESL). A 385 nm light emitting diode (LED) is used as the excitation source and a photomultiplier tube as the light detector. OTC is extracted from honey and cleaned up by solid phase extraction (SPE) using Strata X-WC weak cation exchange cartridges. To the eluate Eu(III) is added to form a Eu-TC chelate at pH 8.5. Efficient intrachelate energy transfer allows sensitive OTC detection at λ_ex=385 nm and λ_em=610 nm. After a 25-µs time delay, the ESL signal is integrated over a 25-1000 µs interval. The signal intensity reveals a linear relationship (R²=0.972) to OTC concentrations in the 10-200 ng/g range. The limit-of-detection is 6.7 ng/g with an average 5.8% relative standard deviation. The background signal corresponds to ~10 ppb. This instrumentation and method combination enables field analysis that is especially useful for beekeeping industry.

Citation Download Citation

Guoying Chen, Daniel Schwartz, S. Braden, and Alberto Nunez "Oxytetracycline analysis in honey using a specific portable analyzer", Proc. SPIE 6761, Optics for Natural Resources, Agriculture, and Foods II, 67610K (22 October 2007); https://doi.org/10.1117/12.735029

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