22 October 2007 Oxytetracycline analysis in honey using a specific portable analyzer
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Oxytetracycline (OTC) residue in honey is detected using a portable analyzer designed to specifically target tetracycline (TC) drugs based on europium-sensitized luminescence (ESL). A 385 nm light emitting diode (LED) is used as the excitation source and a photomultiplier tube as the light detector. OTC is extracted from honey and cleaned up by solid phase extraction (SPE) using Strata X-WC weak cation exchange cartridges. To the eluate Eu(III) is added to form a Eu-TC chelate at pH 8.5. Efficient intrachelate energy transfer allows sensitive OTC detection at λex=385 nm and λem=610 nm. After a 25-µs time delay, the ESL signal is integrated over a 25-1000 µs interval. The signal intensity reveals a linear relationship (R2=0.972) to OTC concentrations in the 10-200 ng/g range. The limit-of-detection is 6.7 ng/g with an average 5.8% relative standard deviation. The background signal corresponds to ~10 ppb. This instrumentation and method combination enables field analysis that is especially useful for beekeeping industry.
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Guoying Chen, Daniel Schwartz, S. Braden, Alberto Nunez, "Oxytetracycline analysis in honey using a specific portable analyzer", Proc. SPIE 6761, Optics for Natural Resources, Agriculture, and Foods II, 67610K (22 October 2007); doi: 10.1117/12.735029; https://doi.org/10.1117/12.735029

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