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12 October 2007 Increased throughput single molecule detection of DNA
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Proceedings Volume 6770, Fiber Optic Sensors and Applications V; 677010 (2007) https://doi.org/10.1117/12.737323
Event: Optics East, 2007, Boston, MA, United States
Abstract
In this work, we present research in using confocal optical techniques with femtolitre focal volumes and obtain very high signal-to-noise and signal-to-background ratios for single molecule detection (SMD). We were able to achieve improved signal strength by using highly fluorescent quantum dots and nanopatterned substrates to obtain plasmon induced resonant fluorescence enhancement. A method to simultaneously using multiple excitation spots without the use of confocal apertures and an array of single photon sensitive Geiger mode avalanche photodiodes was used to increase the throughput of the detection system. Using this highly sensitive SMD system, we detect small quantities of synthetic DNA through hybridization eliminating the need of polymerase chain reaction.
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Rajan Gurjar, Madhavi Seetamraju, Noah Kolodziejski, Richard Myers, Christopher Staples, James Christian, Michael R Squillante, and Gerald Entine "Increased throughput single molecule detection of DNA", Proc. SPIE 6770, Fiber Optic Sensors and Applications V, 677010 (12 October 2007); https://doi.org/10.1117/12.737323
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