Spectrally and time-resolved study of NAD(P)H autofluorescence in cardiac myocytes from human biopsies

Y. Cheng; D. Chorvat Jr.; N. Poirier; J. Miró; N. Dahdah; A. Chorvatova

doi:10.1117/12.732876

15 October 2007 Spectrally and time-resolved study of NAD(P)H autofluorescence in cardiac myocytes from human biopsies

Y. Cheng, D. Chorvat Jr., N. Poirier, J. Miró, N. Dahdah, A. Chorvatova

Proceedings Volume 6771, Advanced Photon Counting Techniques II; 677104 (2007) https://doi.org/10.1117/12.732876
Event: Optics East, 2007, Boston, MA, United States

Abstract

Rejection of transplanted hearts remains an important reason for death of transplanted children. Finding diagnostic tools for its detection can therefore improve the prognosis in this population of patients. Endomyocardial biopsy (EMB) by cardiac catheterization is currently accepted as the "gold standard" for the diagnosis of rejection. Here, we investigate new approach to monitor mitochondrial metabolic state of cardiac cells using spectrally-resolved autofluorescence lifetime detection of nicotinamide adenine dinucleotide (phosphate), or NAD(P)H, the principal electron donor in mitochondrial oxidative energy metabolism responsible for vital ATP supply of cardiomyocytes. NAD(P)H autofluorescence is long used for non-invasive fluorescent probing the metabolic state of the heart. In this contribution we report dynamic characteristics of NAD(P)H fluorescence decays in living human cardiomyocytes from EMB, following excitation by UV-pulsed laser diode and detection by spectrally-resolved time-correlated single photon counting. At least a 3-exponential decay model, with 0.5-0.7 ns, 1.9-2.4 ns and 9.0-15.0 ns lifetimes, is necessary to describe cardiomyocyte autofluorescence in human cells. When gathered data were compared to those recorded under same conditions in rats, autofluorescence in human hearts was found significantly lower in comparison to rat ones. Rotenone, the inhibitor of the Complex I of the respiratory chain, increased the fluorescence in human cardiac cells, making them more comparable to experimental rat model. These results suggest that human cardiac cells are more metabolically active than the rat ones in the same conditions. Presented work proposes a new tool for evaluation of oxidative metabolism changes in transplanted hearts.

Citation Download Citation

Y. Cheng, D. Chorvat Jr., N. Poirier, J. Miró, N. Dahdah, and A. Chorvatova "Spectrally and time-resolved study of NAD(P)H autofluorescence in cardiac myocytes from human biopsies", Proc. SPIE 6771, Advanced Photon Counting Techniques II, 677104 (15 October 2007); https://doi.org/10.1117/12.732876

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