Microscopic image analysis of reticulocyte based on watershed algorithm

J. Q. Wang; G. F. Liu; J. G. Liu; G. Wang

doi:10.1117/12.752933

14 November 2007 Microscopic image analysis for reticulocyte based on watershed algorithm

J. Q. Wang, G. F. Liu, J. G. Liu, G. Wang

Proceedings Volume 6789, MIPPR 2007: Medical Imaging, Parallel Processing of Images, and Optimization Techniques; 67891O (2007) https://doi.org/10.1117/12.752933
Event: International Symposium on Multispectral Image Processing and Pattern Recognition, 2007, Wuhan, China

Abstract

We present a watershed-based algorithm in the analysis of light microscopic image for reticulocyte (RET), which will be used in an automated recognition system for RET in peripheral blood. The original images, obtained by micrography, are segmented by modified watershed algorithm and are recognized in term of gray entropy and area of connective area. In the process of watershed algorithm, judgment conditions are controlled according to character of the image, besides, the segmentation is performed by morphological subtraction. The algorithm was simulated with MATLAB software. It is similar for automated and manual scoring and there is good correlation(r=0.956) between the methods, which is resulted from 50 pieces of RET images. The result indicates that the algorithm for peripheral blood RETs is comparable to conventional manual scoring, and it is superior in objectivity. This algorithm avoids time-consuming calculation such as ultra-erosion and region-growth, which will speed up the computation consequentially.

Citation Download Citation

J. Q. Wang, G. F. Liu, J. G. Liu, and G. Wang "Microscopic image analysis for reticulocyte based on watershed algorithm", Proc. SPIE 6789, MIPPR 2007: Medical Imaging, Parallel Processing of Images, and Optimization Techniques, 67891O (14 November 2007); https://doi.org/10.1117/12.752933

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