8 November 2007 Emission of carbon nanotube-DNA-GOX bionanohybrid for glucose detection
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Proceedings Volume 6796, Photonics North 2007; 679625 (2007) https://doi.org/10.1117/12.778902
Event: Photonics North 2007, 2007, Ottawa, Canada
Emission of individual single-walled carbon nanotubes (SWNTs) in aqueous suspension with single-stranded DNA (ss-DNA) wrapped around the tube with and without glucose oxidase (GOX) has been studied in the spectral range (9-13)•103 cm-1. GOX was added into the aqueous suspension of SWNTs with wrapped ss-DNA, which was prepared before using ultrasonication/ultracentrifugation treatment. AFM images of the nanotube with the adsorbed enzyme show that GOX retains its native globular structure. Computer simulation of SWNT:DNA:GOX bionanohybrid formation in water demonstrates effective coupling of the enzyme with DNA wrapped around the nanotube. GOX adding was not accompanied by a change of Raman spectrum of nanotubes. However, in the spectrum of nanotubes emission a small spectral blue shift was observed after GOX immobilization. Small glucose injections (1 mM in portion) into the prepared aqueous suspension lead to the quenching of the intensity of nanotube luminescence due to the appearance of H2O2 in the solution as a result of glucose oxidizing. The DNA interlayer between the GOX and nanotube keeps the enzyme activity which usually decreases when the enzyme is bound directly to the nanotube.
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V. A. Karachevtsev, V. A. Karachevtsev, A. Yu. Glamazda, A. Yu. Glamazda, M. V. Karachevtsev, M. V. Karachevtsev, Oksana S. Lytvyn, Oksana S. Lytvyn, U. Dettlaff-Weglikowska, U. Dettlaff-Weglikowska, } "Emission of carbon nanotube-DNA-GOX bionanohybrid for glucose detection", Proc. SPIE 6796, Photonics North 2007, 679625 (8 November 2007); doi: 10.1117/12.778902; https://doi.org/10.1117/12.778902

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