9 January 2008 A surface-plasmon resonance phase modulation bio-reaction detection system with (5,1) phase-shifting algorithm
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Surface plasmon resonance (SPR) is a very important metrology in biology detection. Phase modulation is one of the SPR detection technologies and the sample changes can be recognized from the phase variation. It is able to detect very tiny bio sample variation due to its high sensitivity. In this study, the optical system design based on a paraboloidal lens-based surface plasmon resonance instrument will be used to control the SPR critical angle. The charge coupled device camera (CCD camera) will be used to record the images of the bio-reaction and (5,1) phase-shifting algorithm will be adopted to retrieve the phase fringes of the whole spot from the intensity maps. The combination of the angle control SPR system and the (5,1) phase-shifting algorithm will expand the whole spot detection ability from the intensity to phase modulation because the intensity maps are going to be recorded for the (5,1) phase-shifting algorithm calculation. The difference between (5,1) phase-shifting algorithm and Five-Step Algorithm1 is that (5,1) phase-shifting algorithm only needs one image map at one time during the bio reaction and Five-Step Algorithm requires five image maps. Therefore, (5,1) phase-shifting algorithm will reduce the process of experiment and the requirement of the memory. The different concentration alcohols were measured by the optical system to verify the (5,1) phase-shifting algorithm applied in SPR phase modulation measurement and to prove the idea is workable and successful.
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Yi-Hung Chen, Yi-Hung Chen, Shu-Sheng Lee, Shu-Sheng Lee, I-Hung Hsu, I-Hung Hsu, Eddie Tseng, Eddie Tseng, Chih-Kung Lee, Chih-Kung Lee, } "A surface-plasmon resonance phase modulation bio-reaction detection system with (5,1) phase-shifting algorithm", Proc. SPIE 6800, Device and Process Technologies for Microelectronics, MEMS, Photonics, and Nanotechnology IV, 68001Q (9 January 2008); doi: 10.1117/12.769156; https://doi.org/10.1117/12.769156


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