Automated analysis of label-free spinning-disc microarray images

Ganapathy Krishnamurthi; Tim Norwood; Manoj Varma

doi:10.1117/12.766935

3 March 2008 Automated analysis of label-free spinning-disc microarray images

Ganapathy Krishnamurthi, Tim Norwood, Manoj Varma

Proceedings Volume 6812, Image Processing: Algorithms and Systems VI; 68120H (2008) https://doi.org/10.1117/12.766935
Event: Electronic Imaging, 2008, San Jose, California, United States

Abstract

We describe a set of methods to enable fully automated analysis of a novel label-free spinning-disc format microarray system. This microarray system operates in a dual-channel mode, simultaneously acquiring fluorescence as well as interferometric signals. The label-free interferometric component enables the design of robust gridding methods, which account for rotational effects difficult to estimate in traditional microarray image analysis. Printing of microarray features in a Cartesian grid is preferable for commercial systems because of the benefits of using existing DNA/protein printing technologies. The spinning disc operation of the microarray requires spatial transformation of Cartesian microarray features, from the reader/scanner frame of reference to the disc frame of reference. We describe a fast spatial transformation method with no measurable degradation in the quality of transformed data, for this purpose. The gridding method uses frequency-domain information to calculate grid spacing and grid rotation. An adaptive morphological segmentation method is used to segment microarray spots with variable sizes accurately. The entire process, from the generation of the raw data to the extraction of biologically relevant information, can be performed without any manual intervention, allowing for the deployment of high-throughput systems. These image analysis methods have enabled this microarray system to achieve superior sensitivity limits.

Citation Download Citation

Ganapathy Krishnamurthi, Tim Norwood, and Manoj Varma "Automated analysis of label-free spinning-disc microarray images", Proc. SPIE 6812, Image Processing: Algorithms and Systems VI, 68120H (3 March 2008); https://doi.org/10.1117/12.766935

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KEYWORDS

Image segmentation

Image analysis

Proteins

Interferometry

Luminescence

Binary data

Algorithm development

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