Wide-field fluorescence sectioning microscopy using dynamic speckle illumination

Jun Yin; Junle Qu; Yonghong Shao; Lingling Zhao; Haoming Lin; Hanben Niu

doi:10.1117/12.757042

8 January 2008 Wide-field fluorescence sectioning microscopy using dynamic speckle illumination

Jun Yin, Junle Qu, Yonghong Shao, Lingling Zhao, Haoming Lin, Hanben Niu

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 682615 (2008) https://doi.org/10.1117/12.757042
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

In this paper, we present a novel wide-field fluorescence sectioning microscopy using dynamic speckle illumination that provides depth discrimination. Some simple modifications are made to a conventional wide field fluorescence microscope, which allows for the illumination of the sample using dynamic speckle patterns and the acquisition of a sequence of independent wide-field fluorescence images. The full-field fluorescence image of the sample at a particular depth is obtained using specific digital image processing algorithms. Compared with conventional confocal scanning fluorescence microscopy, this novel imaging modality is capable of providing a reasonably high sectioning performance with a compact and cost-effective system. The experimental results show that the wide-field fluorescence sectioning microscopy can provide high contrast imaging even in scattering biological media, therefore has wide potential applications in biomedical research and clinics.

Citation Download Citation

Jun Yin, Junle Qu, Yonghong Shao, Lingling Zhao, Haoming Lin, and Hanben Niu "Wide-field fluorescence sectioning microscopy using dynamic speckle illumination", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 682615 (8 January 2008); https://doi.org/10.1117/12.757042

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