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8 January 2008 Wide-field fluorescence sectioning microscopy using dynamic speckle illumination
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Abstract
In this paper, we present a novel wide-field fluorescence sectioning microscopy using dynamic speckle illumination that provides depth discrimination. Some simple modifications are made to a conventional wide field fluorescence microscope, which allows for the illumination of the sample using dynamic speckle patterns and the acquisition of a sequence of independent wide-field fluorescence images. The full-field fluorescence image of the sample at a particular depth is obtained using specific digital image processing algorithms. Compared with conventional confocal scanning fluorescence microscopy, this novel imaging modality is capable of providing a reasonably high sectioning performance with a compact and cost-effective system. The experimental results show that the wide-field fluorescence sectioning microscopy can provide high contrast imaging even in scattering biological media, therefore has wide potential applications in biomedical research and clinics.
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Jun Yin, Junle Qu, Yonghong Shao, Lingling Zhao, Haoming Lin, and Hanben Niu "Wide-field fluorescence sectioning microscopy using dynamic speckle illumination", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 682615 (8 January 2008); https://doi.org/10.1117/12.757042
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