8 January 2008 Alpha-fetoprotein detection by using a localized surface plasmon coupled fluorescence fiber-optic biosensor
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Abstract
Alpha-fetoprotein (AFP) detection by using a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor is setup and experimentally demonstrated. It is based on gold nanoparticle (GNP) and coupled with localized surface plasmon wave on the surface of GNP. In this experiment, the fluorophores are labeled on anti-AFP which are bound to protein A conjugated GNP. Thus, LSPCF is excited with high efficiency in the near field of localized surface plasmon wave. Therefore, not only the sensitivity of LSPCF biosensor is enhanced but also the specific selectivity of AFP is improved. Experimentally, the ability of real time measurement in the range of AFP concentration from 0.1ng/ml to 100ng/ml was detected. To compare with conventional methods such as enzyme-linked immunosorbent assay (ELISA) or radioimmunoassay (RIA), the LSPCF fiber-optic biosensor performs higher or comparable detection sensitivity, respectively.
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Ying-Feng Chang, Ying-Feng Chang, Ran-Chou Chen, Ran-Chou Chen, Ying-Chang Li, Ying-Chang Li, Chih-Jen Yu, Chih-Jen Yu, Bao-Yu Hsieh, Bao-Yu Hsieh, Chien Chou, Chien Chou, "Alpha-fetoprotein detection by using a localized surface plasmon coupled fluorescence fiber-optic biosensor", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68261B (8 January 2008); doi: 10.1117/12.754617; https://doi.org/10.1117/12.754617
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