8 January 2008 Measurement of intracellular pH (pHi) in a single cell using fluorescent probe and fiber optic nanoprobe
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Abstract
An optical system for measurement of the intracellular pH (pHi) in a single living cell by using the fluorescent probe, 5(6)-carboxyfluorescein diacetate (CFDA) and fiber optic nanoprobe was demonstrated in this work. The CFDA probe is used to determine pHi in the yeast, Saccharomyces cerevisiae 97 while fiber optic nanoprobe is used to guide excitation light and receive emission light within a single cell. Experimental results showed that our system had higher detection sensitivity than other standard spectrometer, which is important to single-cell analysis, especially for the microanalysis in a single-cell.
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Qinmiao Chen, Yishen Qiu, Zhihao Chen, Na Fang, Gaoming Li, "Measurement of intracellular pH (pHi) in a single cell using fluorescent probe and fiber optic nanoprobe", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68262N (8 January 2008); doi: 10.1117/12.755230; https://doi.org/10.1117/12.755230
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