A novel field of view zoom scanning protocol for simultaneous time- and spectrum-resolved multifocal multiphoton microscopy

Junle Qu D.D.S.; Lixin Liu; Ziyang Lin; Lei Wang; Wenqing Liu; Hanben Niu

doi:10.1117/12.760252

8 January 2008 A novel field of view zoom scanning protocol for simultaneous time- and spectrum-resolved multifocal multiphoton microscopy

Junle Qu D.D.S., Lixin Liu, Ziyang Lin, Lei Wang, Wenqing Liu, Hanben Niu

Author Affiliations +

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 682636 (2008) https://doi.org/10.1117/12.760252
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

We present a simultaneous time- and spectrum-resolved multifocal multiphoton microscopy (MMM) system using a novel field of view (FOV) zoom scanning protocol. The system employs a microlens array for producing discrete excitation spot array, a dispersive prism and a high repetition rate streak camera for simultaneous temporal and spectral resolutions. By combining a pair of galvo mirrors and a sample stage for fine and coarse scanning respectively, the resolution and FOV of the system can be changed without changing any optical elements. The system can be operated not only in low resolution and large FOV, but also in high resolution and small FOV applications, without compromising the performances of the optical elements in the system. By implementing a special system control protocol and image reconstruction algorithm, fluorescence images in different FOVs and resolutions can be obtained. This FOV zoom scanning protocol is demonstrated with two-photon excitation fluorescence imaging of a fluorescence resolution test target.

Citation Download Citation

Junle Qu D.D.S., Lixin Liu, Ziyang Lin, Lei Wang, Wenqing Liu, and Hanben Niu "A novel field of view zoom scanning protocol for simultaneous time- and spectrum-resolved multifocal multiphoton microscopy", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 682636 (8 January 2008); https://doi.org/10.1117/12.760252

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