Wavefront coding extended the depth of field to a great extent with simpler structure compared to confocal microscope.
With cubic phase mask (CPM) employed in the STOP of the objective lens, blurred images will be obtained in charge
coupled device (CCD), which will be restored to sharp images by Wiener filter. We proposed that one CPM is used in
one microscope although there are different objective lenses with different power indices. The microscope proposed here
is the wavefront coding one when the CPM is used in the STOP; while it is the traditional one when a plane plate is used
in the STOP. Firstly, make the STOP in the last surface of the lens, and then add a plane plate at the STOP with the same
material and the same center thickness of the CPM. Traditional objective lenses are designed, based on which wavefront
coding system will be designed with the plane plate replaced by a CPM. Secondly, the parameters of CPMs in different
objective lenses are optimized to certain ranges based on metric function of stability of modulation transfer function
(MTF). The optimal parameter is chosen from these ranges. A set of objective lenses is designed as an example with one
CPM. The simulation results shows that the depth of field of 4X, 10X, 40X, 60X and 100X objective lenses with the
same CPM can reach to 400um, 40um, 24um, 16um and 2um respectively, which is much larger than 55.5um, 8.5um,
1um, 0.4um and 0.19um of the traditional ones.